The Lyme PCR is a direct detection test, but has not been rigorously evaluated in children undergoing evaluation for acute Lyme disease.
Published in Vector-Borne and Zoonotic Diseases: https://www.liebertpub.com/doi/10.1089/vbz.2019.2547
PubMed: https://www.ncbi.nlm.nih.gov/pubmed/31821110