A comparison of Lyme disease serologic test results from 4 laboratories in patients with persistent symptoms after antibiotic treatment.

Clin Infect Dis 59:1705-10

YEAR: 2014 TOPICS: Chronic Lyme, Diagnostics.

Diagnosis of Lyme disease in late-stage patients is often erroneous. Fallon and colleagues compared Lyme disease test results from four different testing labs, using blood samples from previously diagnosed Lyme patients and healthy controls. Overall, they found variation in results, but no statistically significant difference between labs. However, there was a higher positivity rate for the C6 test, than for whole bacterial sonicate used in the diagnostic test.

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Anti-neural antibody response in patients with post-treatment Lyme disease symptoms versus those with myalgic encephalomyelitis/chronic fatigue syndrome

Brain Behav Immun 48;354-355

Alaedini A

YEAR: 2015 TOPICS: Chronic Lyme, Diagnostics.

Patients with persisting Lyme symptoms were previously found to have higher and more frequent anti-neural antibodies compared to people who were healthy or who had recovered from Lyme disease. Patients with chronic fatigue syndrome (CFS) and persisting Lyme disease often have similar symptoms. This study found that CFS patients do not have elevated anti-neural antibodies. Thus, the common symptoms of persisting Lyme and CFS are not likely due to anti-neural antibodies

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C-reactive protein response in patients with post-treatment Lyme disease symptoms versus those with myalgic encephalomyelitis/chronic fatigue syndrome

Clin Inf Dis. 67(8):1309–1310. doi.org/10.1093/cid/ciy299

Alaedini A

YEAR: 2018 TOPICS: Basic Science, Diagnostics.

Development of a Multiantigen Panel for Improved Detection of Borrelia burgdorferi Infection in Early Lyme Disease

J Clin Microbiol 53(12):3834-41. Epub 2015 Oct 7

Aucott JN, Soloski MJ

YEAR: 2015 TOPICS: Diagnostics.

The current blood test for Lyme disease has low accuracy. In this study, the authors designed and tested a new blood test that is more accurate. They used portions of proteins, called peptides, from Borrelia burgdorferi, the bacteria that cause Lyme disease. For a patient to be scored as positive, their blood had to contain antibodies that would recognize at least 2 of the 10 bacterial peptides. This test was more sensitive than the current blood test, and could be developed into a newer generation of diagnostic test for Lyme disease.

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Distinct cerebrospinal fluid proteomes differentiate post-treatment Lyme disease from chronic fatigue syndrome

PLoS One. 6(2):e17287

YEAR: 2011 TOPICS: Chronic Lyme, Diagnostics, Treatment.

Chronic fatigue syndrome patients and post-treatment Lyme disease patients share some similar neurological symptoms. This study showed that the protein profiles in cerebrospinal fluid from the two groups were distinctive, suggesting that they are two different diseases.

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Epitope mapping of antibodies to VlsE protein of Borrelia burgdorferi in post-Lyme disease syndrome.

Clin Immunol 141:103-110

Alaedini A

YEAR: 2011 TOPICS: Chronic Lyme, Diagnostics.

The immune response to B. burgdorferi infection includes the production of antibodies that recognize bacterial surface proteins, one of which is called VlsE. Proteins form complex three-dimensional shapes, and only parts of them, called epitopes, are recognized by antibodies. Alaedini’s group mapped these on VlsE. The antibodies against VlsE differ in patients with later compared to earlier stages of Lyme disease.

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Epitope-Specific Evolution of Human B Cell Responses to Borrelia burgdorferi VlsE Protein from Early to Late Stages of Lyme Disease

J Immunol 196:1036-43

Alaedini A

YEAR: 2016 TOPICS: Chronic Lyme, Diagnostics, Treatment.

Lyme disease patients make antibodies that recognize Borrelia burgdorferi, the bacteria that cause illness. When the disease progresses from early to late stages, these antibodies may change. A protein on the outside of B. burgdorferi is called VlsE, and antibodies that bind to a portion of it called the membrane-proximal domain illustrate this. These specific antibodies were low in patients with early disease, and were increased in patients with symptoms typical of later illness, such as neurological symptoms.

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Longitudinal Transcriptome Analysis Reveals a Sustained Differential Gene Expression Signature in Patients Treated for Acute Lyme Disease

mBio. Feb 12;7(1). pii: e00100-16. doi: 10.1128/mBio.00100-16.

Aucott JN, Chiu CY, Soloski MJ

YEAR: 2016 TOPICS: Chronic Lyme, Diagnostics, Treatment.

The genes that are expressed in sick people differ from those who are healthy. Using advanced sequencing techniques, these researchers found that immune cells in the blood of people with Lyme disease express different genes from those of healthy controls. Genes that are linked to a strong immune response were reduced in Lyme patients compared to those with other bacterial or viral infections. People with persisting symptoms attributed to Lyme disease shared some gene expression patterns with patients suffering chronic illnesses caused by immune system dysfunction.

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Serum inflammatory mediator as markers of human Lyme disease activity

PLoS One. 9(4):e93243.

Aucott JN, Soloski MJ

YEAR: 2014 TOPICS: Chronic Lyme, Diagnostics, Treatment.

The authors compared the blood of patients suffering more severe symptoms of Lyme disease against those with less. They found that the pattern of chemokines, which are chemical messengers produced by immune cells, varies between the two groups. This work may lead to better diagnostic tools and clinical treatments, and shows a biological difference between these two groups of patients.

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The Clinical, Symptom, and Quality-of-Life Characterization of a Well-Defined Group of Patients with Posttreatment Lyme Disease Syndrome

Front Med (Lausanne) 4:224. doi: 10.3389/fmed.2017.00224

Aucott JN, Soloski MJ

YEAR: 2017 TOPICS: Chronic Lyme, Diagnostics, Patient Care.

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